H2-formingN5,N10-methylenetetrahydromethanopterin dehydrogenase: mechanism of H2formation analyzed using hydrogen isotopes
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چکیده
منابع مشابه
Quantum thermal rate constants for the exchange reactions of hydrogen isotopes: D+H2
Accurate thermal rate constants for the D + H, reactions are determined for the LiuSiegbahn-Truhlar-Horowitz potential energy surface over the temperature range 300-1500 K. We evaluate the rate constants via the quantum tlux-flux autocorrelation function formulation of Miller [J. Chem. Phys. 61, 1823 ( 1974)] using the adiabatically adjusted principal axis hyperspherical coordinates of Pack [Ch...
متن کاملMeasurement and ab initio calculation of CSA/dipole-dipole cross-correlated relaxation provide insight into the mechanism of a H2-forming dehydrogenase.
Using transferred cross-correlated relaxation and DFT calculations, the conformation of the relevant conformation of N5,N10-methylenetetrahydromethanopterin, a reaction intermediate bound to the 80 kD H2-forming N5,N10-methylenetetrahydromethanopterin dehydrogenase is determined. The conformation of the intermediate differs from the free form in solution and makes the reaction mechanism plausible.
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The iron-sulfur-cluster-free hydrogenase Hmd (H(2)-forming methylenetetrahydromethanopterin dehydrogenase) from methanogenic archaea has recently been found to contain one iron associated tightly with an extractable cofactor of yet unknown structure. We report here that Hmd contains intrinsic CO bound to the Fe. Chemical analysis of Hmd revealed the presence of 2.4 +/- 0.2 mol of CO/mol of iron...
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For large and complex reacting systems, computational efficiency becomes a critical issue in process simulation, optimization and model-based control. Mechanism simplification is often a necessity to improve computational speed. We present a novel approach to simplification of reaction networks that formulates the model reduction problem as an optimization problem and solves it using geneti...
متن کاملStable hydrogen isotopes of long chain alkenones
The effect of temperature and salinity on the stable hydrogen isotopic composition of long chain alkenones produced by Emiliania huxleyi and Gephyrocapsa oceanica S. Schouten, J. Ossebaar, K. Schreiber, M. V. M. Kienhuis, G. Langer, and J. Bijma Royal Netherlands Institute for Sea Research, Department of Marine Biogeochemistry and Toxicology, P.O. Box 59, 1790 AB, Den Burg, Texel, The Netherlan...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1995
ISSN: 0014-5793
DOI: 10.1016/0014-5793(95)00642-m